Expression heterogeneity of ABC-transporter family genes and chemosensitivity genes in gastric tumor, carcinomatosis and lymph node metastases
نویسندگان
چکیده
Introduction . Metastatic tumors (particularly gastric cancer) have been found to be characterized by heterogeneity between the primary tumor and metastases. This type of comes fore when treating primary-metastatic forms is an important reason for low effectiveness their treatment. In this regard, comparative analysis ABC-transporter gene expression chemosensitivity genes will allow characterize a certain extent resistance sensitivity tumor, carcinomatosis metastases therapy provide basis personalized treatment approach. Aim To evaluate in lymph node Materials methods Overall 41 patients with disseminated cancer stage IV peritoneum were included investigation. All underwent surgery according Roux palliative gastrectomy. After chemotherapy depending on indications. RNA was isolated using RNeasy Plus mini kit (Qiagen, Germany). The level ABC transporter (ABCB1, ABCC1, ABCC2, ABCC5, ABCG1, ABCG2) (BRCA1, RRM1, ERCC1, TOP1, TOP2α, TUBβ3, TYMS, GSTP1) assessed reverse transcription polymerase chain reaction (RT-PCR) Results levels under study shown vary widely. For genes, ABCG1 (3.1 ± 1.1; max 32.0), ABCG2 (7.9 2.3; 54.1), (9.6 3.8; 101.0) most expressed tissue, metastasis, respectively. Hyperexpression among at all three sites characteristic only TOP2α (17.2 6.0; max. 161.9; 10.8 4.1; 105.1; 35.3 0.8; 439.6, respectively). We that BRCA1 higher metastasis compared tissue (at p = 0.005 0.001). Whereas ABCC1 statistically significantly (p 0.03). Conclusion Thus, high observed cancer, which affects patterns various different localizations. profile can used determine approach tactics.
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ژورنال
عنوان ژورنال: Uspehi molekulârnoj onkologii
سال: 2022
ISSN: ['2313-805X', '2413-3787']
DOI: https://doi.org/10.17650/2313-805x-2022-9-4-78-88